FastQCFastQC Report
Thu 26 May 2016
SRR1511937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580622
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31840.20143968640193544No Hit
GGTATCAACGCAGAGTACTTTTTTT19130.12102830404739401No Hit
TATCAACGCAGAGTACTTTTTTTTT19110.12090177158106112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCG250.006053514318.98835815
TCGTTGT508.714952E-515.19982219
GGTATCA7200.012.3327651
ATCGTCC550.003084064512.0827358
TCCAACG1603.6379788E-1211.87486118
GTTCTAG1700.011.7945221
AGGACGT6800.011.4619175
GTACTAA1354.3673936E-911.3160851
GGACGTG6800.011.1770496
ATTACAC1553.092282E-1011.0380693
TAGGACG7150.011.0337814
GACGTGA3500.010.8498047
GTATCAA15900.010.8089961
GTACTAG909.040834E-510.60882951
TAACACC909.463714E-510.5611164
TAGACAG2550.010.4368675
TTAGGAC4750.010.4054783
CGTGAAA3800.010.2430729
GGCGAGG3350.010.20883719
GATATAC2900.010.2064251
Produced by FastQC (version 0.11.5)