FastQCFastQC Report
Thu 26 May 2016
SRR1511936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581122
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT44980.28448152640972674No Hit
CTGTAGGACGTGGAATATGGCAAGA42570.2692391858439766No Hit
GTCCTAAAGTGTGTATTTCTCATTT39670.2508977801839453No Hit
CTTTAGGACGTGAAATATGGCGAGG33920.21453119999595224No Hit
GTATCAACGCAGAGTACTTTTTTTT27820.17595100188347262No Hit
CTGAAGGACCTGGAATATGGCGAGA19650.1242788349033155No Hit
GGTATCAACGCAGAGTACTTTTTTT16370.10353407263955597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG602.5485097E-514.26170351
ATCCCGT957.396375E-812.99874510
GGTATCA7850.012.8385621
TAGGACC4500.012.2432624
GTCAACG550.003049675612.100842
CCCGTTT1052.7231908E-711.76076912
TCCCGTT1052.7231908E-711.76076911
GACGTGA11900.011.6543377
GGCGAGG10050.011.62574419
CCAACGA1157.082235E-811.56410119
CCGTTTC1157.082235E-811.56410113
TTAGGAC14050.011.5611613
TCCAACG1501.7644197E-1011.398918
GCGCAGG600.005879959611.08226311
CGTTTCC1053.4697441E-610.85609414
AGGACGT23200.010.8093015
GGACGTG23100.010.6093656
CGTGAAA14300.010.5619179
ACGTGAA14350.010.5251178
TTTAGGA14550.010.2592782
Produced by FastQC (version 0.11.5)