FastQCFastQC Report
Thu 26 May 2016
SRR1511936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581122
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46190.2921343198058088No Hit
CTGTAGGACGTGGAATATGGCAAGA44340.2804337679192371No Hit
GTCCTAAAGTGTGTATTTCTCATTT41870.2648119499950035No Hit
CTTTAGGACGTGAAATATGGCGAGG37830.23926047452378754No Hit
GTATCAACGCAGAGTACTTTTTTTT32780.2073211301847675No Hit
CTGAAGGACCTGGAATATGGCGAGA20660.1306667037711195No Hit
GGTATCAACGCAGAGTACTTTTTTT18930.11972510660151461No Hit
TATCAACGCAGAGTACTTTTTTTTT18880.1194088754694451No Hit
GTCCTACAGTGTGCATTTCTCATTT16830.1064433990545954No Hit
GTCCTTCAGTGTGCATTTCTCATTT16690.1055579518848008No Hit
ATTTAGAAATGTCCACTGTAGGACG16440.10397679622445327No Hit
TTTCTAAATTTTCCACCTTTTTCAG15880.10043500754527482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC400.00529919314.24186510
GGTATCA8000.013.3646791
TAGGACC4900.013.1882424
TATGTCG1001.4514444E-712.34412116
GGCGAGG9700.011.8486219
TCCAACG1201.2778582E-711.08156118
TGTCGAG1201.2778582E-711.08156118
GGCGTGC600.00589937411.0773568
GACGTGA12550.010.9701767
AATCCCG1408.36917E-910.85540819
ACGTGAA14200.010.7653198
GTCGAGG1158.833158E-710.73741419
AGGACGT23300.010.6453185
ATGTCGA1252.2542008E-710.63493517
GGACGTG22700.010.627926
TTAGGAC14300.010.5665973
CCAACGA909.5298215E-510.55386819
AGACGGT909.532879E-510.55353456
GTCTTAA1907.2759576E-1210.5510631
ACCTTTT6050.010.51565315
Produced by FastQC (version 0.11.5)