FastQCFastQC Report
Thu 26 May 2016
SRR1511935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526496
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19340.12669538603442132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.012.6821271
GGCCGTG658.033845E-411.6904747
CGGTTTC600.0058810111.08195813
CCGTGTA600.00588244411.0815949
TTTATAC2400.011.0815943
CGTTTTG600.00588244411.08159418
TTAGGAC4150.010.9862293
TAGGACC3150.010.8554394
GTTCTAG1855.820766E-1110.2828051
GTATTAT1855.820766E-1110.2828051
TAGGACG8050.010.26547054
GTAGGAC8850.010.196143
GGCGAGG3450.010.18680819
TGGTATC2350.010.1187182
AGGACGT7900.010.0996825
TAGGACA3300.010.0741774
ACTATCC1054.1111576E-59.950828
CTAGCAC1151.0280159E-59.9114893
GACGTGA4050.09.85030657
GTTCTAA1553.9844963E-89.8184211
Produced by FastQC (version 0.11.5)