FastQCFastQC Report
Thu 26 May 2016
SRR1511935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526496
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23510.15401285034484205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACCC903.6256097E-813.725943
GGTATCA6050.013.5620351
GGACGTG7900.012.2635166
TAGGACC3050.012.1508314
AGGACGT8300.012.0213285
TAGGACG8500.011.4030894
GACGTGG4100.011.3474337
GACGTGA4300.011.0404557
TTAGGAC5950.010.8600843
ACGTGAA5250.010.8511918
CTAGGAC1855.456968E-1210.7867063
GTATCAA13450.010.4983551
CGTGAAA5250.010.4894859
GTAGGAC8700.010.4855993
TCTTATA2200.010.3664452
GTCCTAA4650.010.2588541
CTTCTCG856.5863144E-410.059918
GTCCTAG1909.094947E-1110.0428781
CCCTTAC951.5743019E-410.0428781
TGTAGGA9300.010.0134652
Produced by FastQC (version 0.11.5)