FastQCFastQC Report
Thu 26 May 2016
SRR1511934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598308
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT29170.18250549956579085No Hit
GTATCAACGCAGAGTACTTTTTTTT28920.18094134547283752No Hit
CTGTAGGACGTGGAATATGGCAAGA27720.1734334058266617No Hit
GTCCTAAAGTGTGTATTTCTCATTT25130.15722876942366554No Hit
CTTTAGGACGTGAAATATGGCGAGG22410.14021077289233366No Hit
TATCAACGCAGAGTACTTTTTTTTT16910.10579938284736108No Hit
GGTATCAACGCAGAGTACTTTTTTT16640.10411009642697151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.012.6815981
CCCGTTT1301.9826984E-1012.4213812
CCGTTTC1405.2750693E-1112.21261713
TAGGACC5250.011.9412254
TCCAACG1850.011.80919618
CGTTTCC1302.6211637E-911.69071114
GGCGAGG7100.011.37168519
AGGACGT16400.010.9466755
TGGCGAG15300.010.86452918
GGACGTG16500.010.82276256
TTAGGAC10300.010.5131263
TGTCGAG1002.4021101E-510.44857118
TCCCGTT1553.6689016E-910.41793111
GACGTGG8250.010.3622197
ATTTCTA10950.010.3227915
GCCGGTT1201.5259993E-610.2902611
GTAGGAC15600.010.229373
GACGTGA9400.010.2060527
TAGGACG18250.010.2013454
CGGTTTC1409.703945E-810.17718113
Produced by FastQC (version 0.11.5)