FastQCFastQC Report
Thu 26 May 2016
SRR1511934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598308
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32000.20021172389802216No Hit
GTCCTACAGTGGACATTTCTAAATT31850.19927323144225015No Hit
CTGTAGGACGTGGAATATGGCAAGA29360.18369425667643532No Hit
GTCCTAAAGTGTGTATTTCTCATTT26310.1646115767424051No Hit
CTTTAGGACGTGAAATATGGCGAGG25000.15641540929532982No Hit
TATCAACGCAGAGTACTTTTTTTTT19960.12488206278139131No Hit
GGTATCAACGCAGAGTACTTTTTTT19680.12313021019728362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGT350.002172696216.2829536
TAACGTG400.005268695814.2542735
CGTAAAG400.005268695814.2542732
GGTATCA7050.012.7274491
TAGGACC4350.012.6704664
GGCGAGG6850.012.06591219
GCACCGA658.0350635E-411.6903256
GTATCAA16200.010.9008251
TGGACCG700.001487714910.8603995
GGACGTG15250.010.8375056
TTAGTAC1158.781226E-710.7423523
AGGACGT15650.010.686915
GTCCTAC15250.010.6409831
ATCCCGT1252.25642E-710.63420110
TTAGGAC9250.010.5815513
GACGTGG7650.010.4256877
GACGTGA8500.010.276757
TGGCGAG15650.010.19826718
GTAGGAC15650.010.140423
TAGGACG16900.010.1213784
Produced by FastQC (version 0.11.5)