FastQCFastQC Report
Thu 26 May 2016
SRR1511933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641339
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT43130.2627732601248127No Hit
CTGTAGGACGTGGAATATGGCAAGA40590.247298090156878No Hit
GTCCTAAAGTGTGTATTTCTCATTT38650.23547847214987275No Hit
CTTTAGGACGTGAAATATGGCGAGG35000.21324053105421853No Hit
GTATCAACGCAGAGTACTTTTTTTT30930.1884437035859137No Hit
CTGAAGGACCTGGAATATGGCGAGA19560.11917099392630041No Hit
GGTATCAACGCAGAGTACTTTTTTT18920.11527173850130899No Hit
GTCCTACAGTGTGCATTTCTCATTT18100.11027581748803872No Hit
TATCAACGCAGAGTACTTTTTTTTT17590.10716859832124868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCC504.5687557E-617.09758811
CGTGCGC500.001501205713.29812310
TGCGCCA802.0027655E-613.060657512
TAGGACC6350.012.1164014
CGGTAGG802.8315932E-511.8892611
GGTATCA9050.011.6659261
GCGCCAC907.4728905E-611.60947313
GGCGAGG9950.011.36020619
GACCTAC1604.5474735E-1111.2947981
AAGGCGT855.3307063E-511.1748936
CCGTCCC600.00588207211.0817699
GCGTGCG600.00588207211.0817699
AAAGGCG951.3623416E-510.9984475
TTGTACT1752.0008883E-1110.8556124
ATTACCC700.001493236210.8556113
AATGTCC6150.010.8114838
TGTCCAC6700.010.77459910
TCCAACG1158.831812E-710.73761518
CGTTTAT803.7293226E-410.70033551
CGTTTCT909.528521E-510.55406714
Produced by FastQC (version 0.11.5)