FastQCFastQC Report
Thu 26 May 2016
SRR1511933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641339
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT47500.2893978635735823No Hit
CTGTAGGACGTGGAATATGGCAAGA42390.25826474603966637No Hit
GTCCTAAAGTGTGTATTTCTCATTT42360.25808196844161996No Hit
GTATCAACGCAGAGTACTTTTTTTT38640.23541754628385725No Hit
CTTTAGGACGTGAAATATGGCGAGG37830.2304825511366025No Hit
TATCAACGCAGAGTACTTTTTTTTT22220.13537727428642102No Hit
GGTATCAACGCAGAGTACTTTTTTT22190.13519449668837455No Hit
GTCCTACAGTGTGCATTTCTCATTT19390.11813525420403707No Hit
CTGAAGGACCTGGAATATGGCGAGA19290.11752599554388216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGT350.002173713916.2818476
GGTATCA7950.013.8100011
CTAGGAC2600.012.4292183
TAGGACC7150.012.3627524
GTATTAA1650.012.1506181
TTATCCG550.003057232612.0968865
ATAGGAC3350.011.6326143
GCGCCAC855.35252E-511.17041313
GTATCAA18500.011.14666751
CCACCTT5750.010.73331113
TAGAAAT6150.010.6638064
GGCGAGG10450.010.63416219
GGTTAGA1355.2894393E-810.6076821
TAGGACA7350.010.6038794
CCTATAC1451.4319085E-810.4879413
AAGGCGT1106.061866E-610.36117656
TTAGGAC12950.010.2753483
GGCGTGC1303.870755E-710.2249138
GGACGTG21750.010.1745966
TGGTATC2250.010.1383422
Produced by FastQC (version 0.11.5)