FastQCFastQC Report
Thu 26 May 2016
SRR1511932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515734
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50780.3350192052167465No Hit
CTGTAGGACGTGGAATATGGCAAGA49980.32974123427989344No Hit
GTCCTAAAGTGTGTATTTCTCATTT44370.2927294630852115No Hit
CTTTAGGACGTGAAATATGGCGAGG38610.25472807233986966No Hit
GTATCAACGCAGAGTACTTTTTTTT26370.1739751170060182No Hit
CTGAAGGACCTGGAATATGGCGAGA22670.1495645014230729No Hit
GTCCTACAGTGTGCATTTCTCATTT19290.12726507421486885No Hit
GTCCTTCAGTGTGCATTTCTCATTT18320.12086553445393453No Hit
ATTTAGAAATGTCCACTGTAGGACG16290.10747268320166994No Hit
CTGTAGGACCTGGAATATGGCGAGA16090.10615319046745669No Hit
GGTATCAACGCAGAGTACTTTTTTT15470.10206276299139559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTTA508.628511E-515.2175191
CGGTAGG953.0013325E-1015.0172881
GTGCGCA456.769139E-414.7757779
CGTTAGG500.001486556413.315331
CAACGAA500.001501048813.29819917
TCCAACG2150.013.25401918
TAGGACC6900.012.9402764
CCAACGA1001.4448597E-712.34832719
GGCGAGG11700.011.69072419
GGCCTAA907.3650463E-611.6244951
CCAACGT1406.82121E-1011.53415319
ATAGGAC2100.011.3079923
GGACGTG25900.011.2957676
GGTATCA6750.011.2722371
AGGACGT26300.011.26843555
GACGTGA13450.011.2289627
GTATCAA15100.011.1485971
CCGTAGG600.005825379411.09610751
CGTAGGA600.005825379411.09610752
GCACCGT600.005881472511.0818326
Produced by FastQC (version 0.11.5)