FastQCFastQC Report
Thu 26 May 2016
SRR1511932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511932_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515734
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT57040.37631932779762145No Hit
CTGTAGGACGTGGAATATGGCAAGA52710.34775231010190444No Hit
GTCCTAAAGTGTGTATTTCTCATTT46230.30500074551339484No Hit
CTTTAGGACGTGAAATATGGCGAGG41630.2746524126264899No Hit
GTATCAACGCAGAGTACTTTTTTTT30970.20432344989292317No Hit
CTGAAGGACCTGGAATATGGCGAGA24050.1586690012891444No Hit
GTCCTACAGTGTGCATTTCTCATTT22000.1451442007634585No Hit
GTCCTTCAGTGTGCATTTCTCATTT19770.13043185677698066No Hit
TATCAACGCAGAGTACTTTTTTTTT18700.12337257064893971No Hit
GGTATCAACGCAGAGTACTTTTTTT18550.12238295109827978No Hit
CTGTAGGACCTGGAATATGGCGAGA18020.11888629535261465No Hit
ATTTAGAAATGTCCACTGTAGGACG17760.1171709547981374No Hit
TTTCTAAATTTTCCACCTTTTTCAG16280.10740670856495928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6450.013.8518444
CCAACGT1001.0075382E-813.29958319
ACCGTGC500.0015058513.2925648
AATCCCG2000.012.82459819
GGTATCA6850.012.3962811
TCCAACG1850.012.32393918
AAGGCGT550.0030706212.0897336
GGCGAGG11150.011.33148419
AGGACCT15100.011.2671885
GTCCTAT2300.011.2002541
GGACCTG14000.011.1274896
GGACGTG24700.011.1143046
AGGACGT25250.011.0668845
TGTAGGA22800.011.0054682
CGGTTTC1658.185452E-1110.93363913
GCGCCAC700.001497926810.8514313
ATCCCGT700.00149916410.85035610
CTGTAGG22500.010.81308651
GTAGGAC22700.010.7189833
GACGTGG12250.010.6960577