FastQCFastQC Report
Thu 26 May 2016
SRR1511931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601773
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42290.26401993291184206No Hit
GTCCTACAGTGGACATTTCTAAATT31290.19534603217809263No Hit
CTGTAGGACGTGGAATATGGCAAGA30530.19060128994557907No Hit
GTCCTAAAGTGTGTATTTCTCATTT25710.16050963526042702No Hit
CTTTAGGACGTGAAATATGGCGAGG25590.15976046543424066No Hit
GGTATCAACGCAGAGTACTTTTTTT25020.15620190875985548No Hit
TATCAACGCAGAGTACTTTTTTTTT23270.14527651546130443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2100.012.66493918
GGTATCA7600.012.6398511
CCAACGA1457.2759576E-1212.44657719
GCCGGTT853.9495426E-612.2924411
CGGTTTC907.4721847E-611.60952813
TGCCGGT907.4721847E-611.60952810
GGCGAGG6800.011.59400719
TGGTATC2800.011.5489232
TAGGACC5350.011.5404824
GTATCAA18500.011.5162331
ACCGTCT600.005881758411.0818228
CCGGTTT951.3622135E-510.998512
ATCCCGT1408.365532E-910.85566210
GTTCTAG1451.41662895E-810.4950891
CGTTTCC1451.4459147E-810.48132814
GTGGTAT3750.010.3988851
CTTAGAC1752.3646862E-1010.312883
ACTGTTC5150.010.1442478
GACCTAC1606.0499588E-910.1056231
TGGCGAG16650.010.04067318
Produced by FastQC (version 0.11.5)