FastQCFastQC Report
Thu 26 May 2016
SRR1511931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601773
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50790.31708612893337573No Hit
GTCCTACAGTGGACATTTCTAAATT33470.2089559506871448No Hit
CTGTAGGACGTGGAATATGGCAAGA32780.20464822418657325No Hit
GGTATCAACGCAGAGTACTTTTTTT29570.1846079313360882No Hit
TATCAACGCAGAGTACTTTTTTTTT28750.1794886041904814No Hit
CTTTAGGACGTGAAATATGGCGAGG28410.17736595634962007No Hit
GTCCTAAAGTGTGTATTTCTCATTT26810.16737702533380197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCA350.00217799516.27701812
GGCGTGC500.001505275713.2933148
GGTATCA9400.013.0948571
CCAACGA751.4805992E-512.66504319
TCCAACG1251.4133548E-912.15844118
TAGGACC5400.011.9677824
GGCGAGG6900.011.42607119
AGGACGA1001.9201816E-611.40459255
GTATCAA21450.010.9432611
TGGTATC3400.010.9014492
AATGCCG2050.010.6571718
TAATACC1451.43372745E-810.4869824
GTCCTAT2100.010.450751
ATGCCGG2100.010.40342819
AGGACCT11200.010.3523835
GAAATGT5450.010.1087966
AAGGCGT856.600952E-410.0575346
AATGTCC5400.010.0227368
GTCCTAA8200.09.8910871
TGTAGGA18300.09.8673622
Produced by FastQC (version 0.11.5)