FastQCFastQC Report
Thu 26 May 2016
SRR1511930_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511930_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468577
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29470.20067044492730035No Hit
GTCCTACAGTGGACATTTCTAAATT27710.18868605459570728No Hit
CTGTAGGACGTGGAATATGGCAAGA25770.17547598798020123No Hit
GTCCTAAAGTGTGTATTTCTCATTT19850.13516485686484264No Hit
GGTATCAACGCAGAGTACTTTTTTT18830.12821935792266936No Hit
CTTTAGGACGTGAAATATGGCGAGG18460.12569991222795945No Hit
TATCAACGCAGAGTACTTTTTTTTT16970.11555403632223574No Hit
GTCCTACAGTGTGCATTTCTCATTT15100.10282062159491806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA350.002171916416.28377
TAGACGT456.768357E-414.775954
GCGGTCC400.005283253314.24823814
GGTATCA7550.014.1077831
ACCGTTC551.9620938E-413.8164748
TAGGACC5200.012.969554
ACTAGAC905.412385E-712.6651013
TACACCG701.09208144E-412.2127755
CACACCG550.00307115112.0894155
CCAACGA1201.0028089E-811.87353219
CTAGGAC2250.011.8207613
TCCAACG1751.8189894E-1211.3985918
ACACCGT600.00588080611.0819646
GTAGGAC14400.010.9500353
GTTACAA4000.010.92364915
GGCGAGG5100.010.80258619
GTCCTAA8150.010.73539451
TGTAGGA14950.010.6866372
TAGGACA4300.010.603344
TCTATAC1451.4450052E-810.48146253
Produced by FastQC (version 0.11.5)