FastQCFastQC Report
Thu 26 May 2016
SRR1511930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468577
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36590.2491527512687452No Hit
GTCCTACAGTGGACATTTCTAAATT30810.2097949239297633No Hit
CTGTAGGACGTGGAATATGGCAAGA26660.18153627627288185No Hit
GGTATCAACGCAGAGTACTTTTTTT21750.14810255097281244No Hit
GTCCTAAAGTGTGTATTTCTCATTT21300.14503836026303013No Hit
TATCAACGCAGAGTACTTTTTTTTT20800.14163370391882754No Hit
CTTTAGGACGTGAAATATGGCGAGG19980.1360500675143353No Hit
GTCCTACAGTGTGCATTTCTCATTT16370.11146844870919263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGCG400.005299865314.2414969
TCCGATT400.005299865314.24149612
TCCAACG1650.013.816051518
TAGGACC5200.012.429634
CCAACGA1001.4450961E-712.34809519
GGTATCA7450.012.0396821
CCAACGT907.4724794E-611.60932119
TGTAGGA14950.011.444162
CCTAGCC1104.9331175E-711.2331943
GACCGAT600.005901557411.076727
CTGTAGG14800.010.9605081
GTAGGAC14400.010.9571643
GTCCTAG2100.010.9052451
GTCCTAC14100.010.6925531
AGGACGT13300.010.6484715
GGACGTG13150.010.619226
GTCCTAA6950.010.5718111
GTATCAA16350.010.5634121
TGGACTG2450.010.474915
GGCGAGG5200.010.41185619
Produced by FastQC (version 0.11.5)