FastQCFastQC Report
Thu 26 May 2016
SRR1511929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521919
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT34980.22984140417459797No Hit
CTGTAGGACGTGGAATATGGCAAGA34110.22412493700387473No Hit
GTCCTAAAGTGTGTATTTCTCATTT30240.1986965140720367No Hit
CTTTAGGACGTGAAATATGGCGAGG26760.17583064538914359No Hit
GTATCAACGCAGAGTACTTTTTTTT25580.1680772761231051No Hit
GTCCTACAGTGTGCATTTCTCATTT16240.10670738718683451No Hit
TATCAACGCAGAGTACTTTTTTTTT15460.10158227868894468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTACG400.00523900614.2663582
ACCGCCC400.005283761314.2480788
GGTCTCG400.005283761314.24807811
GTCCCGT500.001501047613.2982066
CGGTAGG957.2763214E-813.0149231
CCAACGA701.0922463E-412.21263819
GGTATCA6600.011.8165791
GTCTAGG1052.679335E-711.7754071
GCATAAT1302.566594E-911.70572951
GACGTGG9500.011.4984487
TAGGACC6700.011.4835264
GGCGAGG7650.011.42329619
GGACGTG18150.011.2519256
AGGACGT18900.011.1572255
GTATAAG1900.011.0126281
ATAAGAC951.3620669E-510.9985173
GTCTAGA2000.010.9375411
TTAGGAC10600.010.8428763
TAGGACG20450.010.77605154
TGTAGGA19200.010.6997682
Produced by FastQC (version 0.11.5)