FastQCFastQC Report
Thu 26 May 2016
SRR1511929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521919
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT38680.2541528162799728No Hit
CTGTAGGACGTGGAATATGGCAAGA35400.23260107798115406No Hit
GTCCTAAAGTGTGTATTTCTCATTT34260.22511053479193047No Hit
GTATCAACGCAGAGTACTTTTTTTT29640.19475412291981373No Hit
CTTTAGGACGTGAAATATGGCGAGG27820.18279553642473745No Hit
GTCCTACAGTGTGCATTTCTCATTT18640.12247695179572632No Hit
GGTATCAACGCAGAGTACTTTTTTT17700.11630053899057703No Hit
TATCAACGCAGAGTACTTTTTTTTT17160.11275238695357638No Hit
CTGAAGGACCTGGAATATGGCGAGA16970.11150396308870576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6250.013.83791354
ACGTCAA500.001505475113.2930098
GGCGAGG9600.012.27043119
ACGAAAT1251.4224497E-912.15280912
GAAGCGA550.0030691512.0905126
CGTCAAA550.003080502412.0845539
CGAAATC1353.7471182E-1011.95667413
TTAGGAC11650.011.8290893
GGTATCA7750.011.5720331
ATAGGAC1900.011.5049163
CCTACAC2750.011.4048743
GTCGAGG1104.9694245E-711.22690419
TTTAGGA13150.011.0579582
TCCAACG1658.0035534E-1110.93903418
AGGACGT19400.010.9247725
TAGAAAT4700.010.91955954
TCCCGTT1053.4900986E-610.85072311
GTATATG2200.010.8417871
GGACGTG18600.010.82760056
TATGTCG1158.870302E-710.73383716
Produced by FastQC (version 0.11.5)