FastQCFastQC Report
Thu 26 May 2016
SRR1511928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563982
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA34700.22186956115863224No Hit
GTCCTACAGTGGACATTTCTAAATT34420.22007925922421104No Hit
GTCCTAAAGTGTGTATTTCTCATTT32910.21042441664929648No Hit
CTTTAGGACGTGAAATATGGCGAGG29350.18766200634022642No Hit
GTATCAACGCAGAGTACTTTTTTTT26560.16982292635081478No Hit
TATCAACGCAGAGTACTTTTTTTTT15740.100640544456394No Hit
CTGAAGGACCTGGAATATGGCGAGA15740.100640544456394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8300.012.3759841
GGCGAGG8900.011.95342619
CGTTAGG752.0513136E-411.4134061
TGGTATC2450.011.2577622
TAGGACC5500.011.0530394
CTAATAG1553.1286618E-1011.0307543
GTCTAAG1855.456968E-1210.7964661
AGGACGT20850.010.7059765
GGACGTG20650.010.5796736
ACCTTTT5450.010.4572915
CTAGACC1553.6689016E-910.4179344
TGTAGGA19350.010.272722
GTAGGAC19450.010.25567153
GTCCCGG750.00262189610.145251
TAGGACG22600.010.129154
GACGTGA10900.010.1087147
CGGTAGG856.522129E-410.0706531
GACGTGG10850.010.0677527
TAGCACT1803.947207E-1010.0264114
ACGTGAA11800.09.981698
Produced by FastQC (version 0.11.5)