FastQCFastQC Report
Thu 26 May 2016
SRR1511928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563982
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT38030.24316136630728488No Hit
CTGTAGGACGTGGAATATGGCAAGA36110.2308850101855392No Hit
GTCCTAAAGTGTGTATTTCTCATTT34900.22314834825464744No Hit
GTATCAACGCAGAGTACTTTTTTTT31990.20454199600762668No Hit
CTTTAGGACGTGAAATATGGCGAGG30940.19782836375354707No Hit
TATCAACGCAGAGTACTTTTTTTTT18730.11975841154182082No Hit
GGTATCAACGCAGAGTACTTTTTTT18260.11675326186618515No Hit
CTGAAGGACCTGGAATATGGCGAGA17350.11093478057931612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7700.012.2662231
ACTATAC1950.012.1827473
GACCGTG802.8810866E-511.8690657
TGGTATC2900.011.1409142
GTCCTAA10150.010.9033091
ACATCGT700.001492458510.8562296
GTATCAA16550.010.3762611
GTCCTAC18500.010.365511
GGACCTG12800.010.3155386
ATAGGCC1201.518023E-610.2944223
GTCTTAT1409.1011316E-810.2218521
AGGACCT12300.010.1205955
ATAGGAC1803.9108272E-1010.0304633
GTGGTAT3350.09.9675771
CTATACT2201.8189894E-129.9344784
AAGGACC7450.09.8214214
GTAGAAC4850.09.7964363
GAACAGT3700.09.7559366
TAGCACT1951.6189006E-109.7461984
TGGCGAG18850.09.72721718
Produced by FastQC (version 0.11.5)