FastQCFastQC Report
Thu 26 May 2016
SRR1511927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378726
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT39140.2838852679937856No Hit
CTGTAGGACGTGGAATATGGCAAGA36970.2681461000953054No Hit
GTCCTAAAGTGTGTATTTCTCATTT34560.2506661947333988No Hit
CTTTAGGACGTGAAATATGGCGAGG30320.2199131662128661No Hit
GTATCAACGCAGAGTACTTTTTTTT22740.16493487465964957No Hit
GTCCTACAGTGTGCATTTCTCATTT19550.14179757254160727No Hit
CTGAAGGACCTGGAATATGGCGAGA16850.12221427607806049No Hit
CTGTAGGACCTGGAATATGGCGAGA14640.1061849852690092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG704.3846558E-714.9436041
GGCGAAA456.767715E-414.77602919
TCCAACG1900.014.49828318
CGGACAC655.4576747E-513.15228819
TAGGACC6450.012.9597064
GCGTGCG853.947618E-612.2926649
GGCGAGG8400.012.09975819
CGGTTTC1201.0024451E-811.87359413
AGGCGTG1052.7229908E-711.7605127
GTCTAGA1701.8189894E-1211.7471121
TAGAAAT6150.011.5839954
GGTATCA4500.011.411481
CCAACGA1501.7644197E-1011.39865119
GTATCAA11350.011.3947241
ATTTAGA5500.011.238581
CGTAGGA855.2673262E-511.1877262
GGCGTGC855.3280335E-511.1751498
AAGGCGT855.3280335E-511.1751496
AGGACGG600.00588026611.0820215
CCCTATA951.3446874E-511.0110782
Produced by FastQC (version 0.11.5)