FastQCFastQC Report
Thu 26 May 2016
SRR1511927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378726
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT44220.3207308776363106No Hit
CTGTAGGACGTGGAATATGGCAAGA39980.2899778491157779No Hit
GTCCTAAAGTGTGTATTTCTCATTT36550.2650998095343092No Hit
CTTTAGGACGTGAAATATGGCGAGG32810.23797331739591476No Hit
GTATCAACGCAGAGTACTTTTTTTT26010.188652422598834No Hit
GTCCTACAGTGTGCATTTCTCATTT22020.15971266226937042No Hit
CTGAAGGACCTGGAATATGGCGAGA16870.122359337533346No Hit
GGTATCAACGCAGAGTACTTTTTTT16250.11786243241949453No Hit
CTGTAGGACCTGGAATATGGCGAGA15820.11474361113085559No Hit
TATCAACGCAGAGTACTTTTTTTTT15310.11104454402107453No Hit
GTCCTTCAGTGTGCATTTCTCATTT13940.10110783433401561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6200.013.5478551
CCAACGA852.704037E-713.409409519
TCCAACG1251.03682396E-1012.91726218
GACACGG604.1088715E-412.6603097
GTAATAC853.927773E-612.2982043
CTAGGAC2000.011.8789463
TAGGACC6100.011.844
GGCGAGG9650.011.41768319
GATATAC4200.011.3631911
CTAATTC600.005860568511.0870183
AACCGTG600.00589708611.077777
AGGACGT20650.011.0448335
GGACGTG19950.010.950066
TTAGGAC12000.010.9286323
AGGACCT11550.010.8607525
GACGTGA11150.010.8151967
GGACCTG11050.010.74432856
AATCCCG1605.456968E-1010.68562319
TGTAGAA5200.010.5996762
TATGTCG909.557397E-510.55063916
Produced by FastQC (version 0.11.5)