FastQCFastQC Report
Thu 26 May 2016
SRR1511926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492118
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT32930.22069300149183912No Hit
CTGTAGGACGTGGAATATGGCAAGA31240.20936681951427435No Hit
GTCCTAAAGTGTGTATTTCTCATTT27450.1839666836000906No Hit
GTATCAACGCAGAGTACTTTTTTTT26510.1776669137427469No Hit
CTTTAGGACGTGAAATATGGCGAGG23710.15890164182725497No Hit
GGTATCAACGCAGAGTACTTTTTTT15290.1024717884242399No Hit
TATCAACGCAGAGTACTTTTTTTTT15140.10146650600019569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG504.514073E-617.1189941
AGACCGG400.00528354514.248146
TAGGACC5100.013.4100154
GTCCTAT1650.012.6807361
AAGACCG550.003071346812.0893335
GGTATCA6250.011.8691691
TCGAACT752.0762242E-411.39851319
AGGACCT10800.011.345745
ACCGTGG855.329377E-511.1750138
TGGACCG600.005881181511.0818875
GGACCTG9900.011.0339146
TGTAGGA16000.010.639932
TCCAACG909.5261734E-510.55417718
GTCTTAG1909.094947E-1210.51166251
ATAAGAC1451.445369E-810.4813913
GTAGGAC16400.010.4254683
TAGTACC750.00265033310.1320114
TAAGACG856.6005293E-410.0575114
GTATCAA12700.010.0347561
CTAGGGC951.645889E-49.9986943
Produced by FastQC (version 0.11.5)