##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511926_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1492118 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.09956786259532 33.0 31.0 34.0 28.0 34.0 2 29.410694730577607 33.0 30.0 34.0 16.0 34.0 3 30.739958904054504 33.0 31.0 34.0 25.0 34.0 4 34.73201047102173 37.0 35.0 37.0 32.0 37.0 5 34.97568154797409 37.0 35.0 37.0 33.0 37.0 6 35.174899706323494 37.0 35.0 37.0 33.0 37.0 7 35.205186185006816 37.0 36.0 37.0 33.0 37.0 8 35.22979281799429 37.0 36.0 37.0 33.0 37.0 9 36.99880505429195 39.0 38.0 39.0 34.0 39.0 10 36.927044643922265 39.0 38.0 39.0 34.0 39.0 11 36.951483059650776 39.0 38.0 39.0 34.0 39.0 12 36.88583677698413 39.0 38.0 39.0 34.0 39.0 13 36.88410232970851 39.0 38.0 39.0 34.0 39.0 14 38.32220306973041 41.0 39.0 41.0 34.0 41.0 15 38.29869420514999 41.0 39.0 41.0 34.0 41.0 16 38.22649080032544 41.0 39.0 41.0 34.0 41.0 17 38.20977563436672 41.0 39.0 41.0 34.0 41.0 18 38.175939168349956 41.0 39.0 41.0 34.0 41.0 19 38.20303354024279 41.0 39.0 41.0 34.0 41.0 20 38.14439273569516 41.0 39.0 41.0 34.0 41.0 21 38.086511924660115 41.0 39.0 41.0 34.0 41.0 22 38.02023097368975 41.0 39.0 41.0 34.0 41.0 23 37.94081902369652 41.0 39.0 41.0 34.0 41.0 24 37.891643288265406 41.0 39.0 41.0 34.0 41.0 25 37.81017453043258 40.0 39.0 41.0 34.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.388120911903318 1101 2 2.007708128576393 1101 3 1.0521415487412362 1101 4 0.3699704266419914 1101 5 0.15262698541335595 1101 6 0.08594687321090788 1101 7 0.01024418003175498 1101 8 -0.047922392776712286 1101 9 -0.158275925688784 1101 10 -0.20871288876701755 1101 11 0.03856195921742511 1101 12 -0.17129185754721732 1101 13 0.05983097870008436 1101 14 -0.1338651361292591 1101 15 -0.07147367616278899 1101 16 -0.0571870002115773 1101 17 -0.06507324565556871 1101 18 -0.08060926986970429 1101 19 0.042815544304993125 1101 20 -0.1561600482265959 1101 21 -0.09701675042954605 1101 22 -0.02522648912839287 1101 23 0.16398097441825854 1101 24 0.06765333894576742 1101 25 -0.17679074956198093 1102 1 0.4342202026834592 1102 2 2.037495362618948 1102 3 0.9407940310107392 1102 4 0.3040129798334803 1102 5 0.21929365208001883 1102 6 -0.10128716934228521 1102 7 -0.04011042989732516 1102 8 -0.035865655188061396 1102 9 -0.10863053561786273 1102 10 -0.059067498696094844 1102 11 -0.22952314716555122 1102 12 -0.24221384336281915 1102 13 -0.24087824115807166 1102 14 -0.23173747655479104 1102 15 -0.31899140665924364 1102 16 -0.17349905694916856 1102 17 -0.03244913218038903 1102 18 -0.13238231951509505 1102 19 -0.03378020037585827 1102 20 -0.1377203319145366 1102 21 -0.11900256603238546 1102 22 -0.2798364182064077 1102 23 -0.31545164969521267 1102 24 -0.18057361140883899 1102 25 -0.14984039495205081 1103 1 0.9140265087276696 1103 2 2.3928164510807086 1103 3 1.3303962343118094 1103 4 0.7949133722641619 1103 5 0.5317887224524753 1103 6 0.3911299321013715 1103 7 0.47861504318529313 1103 8 0.5879840445087225 1103 9 0.5238228996052143 1103 10 0.4535665710104553 1103 11 0.6212465212402236 1103 12 0.5225902322269533 1103 13 0.8170489231908675 1103 14 0.8336510427467232 1103 15 0.7673944167016913 1103 16 0.8280848889509969 1103 17 0.7039292563324508 1103 18 0.9336229689246878 1103 19 0.7742138776060017 1103 20 0.8345043685903875 1103 21 0.8918264518565024 1103 22 0.9195565102832788 1103 23 0.9358339325180438 1103 24 0.875408855279808 1103 25 0.9563925937922377 1104 1 0.7422520325095832 1104 2 2.464995982396413 1104 3 1.1833311812010514 1104 4 0.860859940979509 1104 5 0.596247652439736 1104 6 0.49160021587736225 1104 7 0.3655220104703645 1104 8 0.3287987656967388 1104 9 0.2816882973533268 1104 10 0.32328853064490204 1104 11 0.20474641471253818 1104 12 0.2779189591493392 1104 13 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25 0.22912148269202248 1106 1 0.622345948646629 1106 2 2.2594200215719837 1106 3 1.1480706859392775 1106 4 0.6223875939054295 1106 5 0.3835002431886352 1106 6 0.21205385789215825 1106 7 0.15542612106490594 1106 8 0.003194702479696332 1106 9 0.004711478014151282 1106 10 0.24149178873029342 1106 11 0.21696971624797357 1106 12 0.2942947037721879 1106 13 0.200386715095199 1106 14 0.11950587759153564 1106 15 0.22825999649131035 1106 16 0.36101006229643673 1106 17 0.2165975145610446 1106 18 0.29315452736641134 1106 19 0.14821212545098916 1106 20 0.29544728662578734 1106 21 0.23301806940493464 1106 22 0.12674186723732106 1106 23 0.08635444426901984 1106 24 0.010436361070667033 1106 25 -0.12423252744497404 1107 1 0.8886975134140123 1107 2 2.6417449157527777 1107 3 1.4509133875234532 1107 4 0.7928413255637494 1107 5 0.5917897600198216 1107 6 0.4169709174939058 1107 7 0.28189844976594003 1107 8 0.2709757217363489 1107 9 0.4135109623928628 1107 10 0.211390322903398 1107 11 0.27947183640129936 1107 12 0.33030244562985445 1107 13 0.40554971248317173 1107 14 0.4259716856432192 1107 15 0.42865029316200776 1107 16 0.42341324196105745 1107 17 0.4315103903935622 1107 18 0.47516827726747124 1107 19 0.41212881190575246 1107 20 0.3616281083783761 1107 21 0.4298644119990982 1107 22 0.5354348728043732 1107 23 0.41878694800990957 1107 24 0.5380765644546202 1107 25 0.4805407386465177 1108 1 0.8757130893351821 1108 2 2.5754731695363056 1108 3 1.3913601379154095 1108 4 0.7471978422899497 1108 5 0.590868458560287 1108 6 0.4274891091569373 1108 7 0.34718424178887375 1108 8 0.26091818080308116 1108 9 0.28767249724876365 1108 10 0.35696538203371375 1108 11 0.41024815410483484 1108 12 0.21507645738587655 1108 13 0.455073706729074 1108 14 0.490043891971645 1108 15 0.5016041688240236 1108 16 0.47332919340685464 1108 17 0.46835032638735186 1108 18 0.4594778668842423 1108 19 0.45378941737860856 1108 20 0.5147365579519345 1108 21 0.46826813599657413 1108 22 0.4941931631705785 1108 23 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-0.23060064917122958 2314 17 -0.19395399338934993 2314 18 -0.2958434063593032 2314 19 -0.1813402022545887 2314 20 -0.20627512032278617 2314 21 -0.2869960844204371 2314 22 -0.12185886900721954 2314 23 -0.09784561897801325 2314 24 -0.13488059558055454 2314 25 -0.16179849931539536 2315 1 0.04398192415009916 2315 2 0.03003126059839545 2315 3 0.016243941196059097 2315 4 -0.08056864739282332 2315 5 -0.15771676493530862 2315 6 -0.2175047626063673 2315 7 -0.13726685620279966 2315 8 -0.24050849772042682 2315 9 -0.38537504167430825 2315 10 -0.37258321347590595 2315 11 -0.5150464338993856 2315 12 -0.46619789614335616 2315 13 -0.3318235081289558 2315 14 -0.3793195463461885 2315 15 -0.35186060450071466 2315 16 -0.40313827342300357 2315 17 -0.5893232093949834 2315 18 -0.5165641505444825 2315 19 -0.38025994837408916 2315 20 -0.3151524874296783 2315 21 -0.330381323651757 2315 22 -0.5916049306298419 2315 23 -0.48527354513828413 2315 24 -0.6743155922409372 2315 25 -0.6485934027211115 2316 1 -0.7333163298833831 2316 2 -2.515987883007412 2316 3 -1.0408739503600835 2316 4 -0.69874609792857 2316 5 -0.5212716346875723 2316 6 -0.46654368072977803 2316 7 -0.6333867086722336 2316 8 -0.6688672508426947 2316 9 -0.7807253095787985 2316 10 -0.7563725352736839 2316 11 -0.7018855444655472 2316 12 -0.6562909030359805 2316 13 -0.7896465614704269 2316 14 -0.8544400760670854 2316 15 -0.8456608107920971 2316 16 -0.7719740001287363 2316 17 -0.7912978115330489 2316 18 -0.8253484721864623 2316 19 -0.7349868466556089 2316 20 -0.7649545185448972 2316 21 -0.91019512876899 2316 22 -0.957850962796492 2316 23 -0.8819173287128947 2316 24 -0.9143427781745572 2316 25 -0.8064158967908384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25079.0 3 0.0 4 231.0 5 1222.0 6 1463.0 7 1329.0 8 1196.0 9 1163.0 10 1127.0 11 1101.0 12 1013.0 13 1068.0 14 1133.0 15 1169.0 16 1221.0 17 1240.0 18 1499.0 19 1745.0 20 2159.0 21 2716.0 22 3386.0 23 3946.0 24 4817.0 25 5405.0 26 6072.0 27 6962.0 28 7950.0 29 9743.0 30 12221.0 31 15771.0 32 21228.0 33 28296.0 34 41024.0 35 62128.0 36 118727.0 37 215538.0 38 880030.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.96932438038546 18.78993121760851 12.631618453760044 25.609125948245993 2 16.39028220002617 20.94628100559971 40.13635250343062 22.527084290943495 3 18.716750283824737 24.412881554944047 29.62868888385503 27.241679277376186 4 12.06908568893345 16.685007486003116 38.804973869358854 32.440932955704575 5 12.906922661405229 37.68177230426094 36.36612215956689 13.045182874766942 6 31.573910374380574 37.54187001296144 18.18508991916189 12.699129693496092 7 27.799812079205534 31.528337571157238 22.574421057851993 18.097429291785232 8 24.50114535177513 38.159113421324584 20.51071027894577 16.829030947954518 9 26.08975965707806 15.639513764997137 20.459373856491244 37.81135272143356 10 14.572104887146994 29.22382814227829 34.95990263504629 21.244164335528424 11 34.253055053286666 22.428454049880774 25.016587159996728 18.301903736835825 12 23.18636025494099 24.572853207873415 32.27758007117438 19.96320646601122 13 28.92284658451946 22.08364217843361 25.902375013236217 23.091136223810718 14 20.458636649380278 22.33389048319235 27.818108219323136 29.389364648104237 15 22.38951611065613 31.60467201655633 23.40505241542559 22.600759457361953 16 21.76041036968926 28.401842213551475 28.177396157676537 21.660351259082727 17 21.25198963879888 28.98127142526833 28.25270339553852 21.514035540394275 18 21.18418248422712 27.77950537423984 30.29907822303598 20.737233918497065 19 23.248529089479746 27.111131068029486 27.670676890268297 21.969662952222468 20 23.253723901192803 27.68534392052103 28.3327458016055 20.728186376680664 21 22.60096051384676 27.04524709171795 27.333226996792483 23.020565397642816 22 22.82647887097401 28.37845264248538 27.663964914303023 21.131103572237585 23 21.880610758851052 28.09945104620233 28.11821640615209 21.901721788794536 24 22.914355911768315 26.952645134396295 28.815099620204045 21.317899333631342 25 22.45311697868399 27.605323439567115 28.35198020531888 21.589579376430017 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 59.0 1 59.0 2 151.0 3 243.0 4 243.0 5 243.0 6 721.5 7 1200.0 8 1200.0 9 1200.0 10 1844.5 11 2489.0 12 2489.0 13 2489.0 14 4504.5 15 6520.0 16 6520.0 17 6520.0 18 11869.0 19 17218.0 20 17218.0 21 17218.0 22 27629.0 23 38040.0 24 38040.0 25 38040.0 26 55587.0 27 73134.0 28 73134.0 29 73134.0 30 95222.0 31 117310.0 32 117310.0 33 117310.0 34 132718.5 35 148127.0 36 148127.0 37 148127.0 38 159684.5 39 171242.0 40 171242.0 41 171242.0 42 179862.0 43 188482.0 44 188482.0 45 188482.0 46 191878.0 47 195274.0 48 195274.0 49 195274.0 50 188127.0 51 180980.0 52 180980.0 53 180980.0 54 159542.5 55 138105.0 56 138105.0 57 138105.0 58 118063.5 59 98022.0 60 98022.0 61 98022.0 62 79379.5 63 60737.0 64 60737.0 65 60737.0 66 46610.5 67 32484.0 68 32484.0 69 32484.0 70 23500.0 71 14516.0 72 14516.0 73 14516.0 74 9996.0 75 5476.0 76 5476.0 77 5476.0 78 3617.5 79 1759.0 80 1759.0 81 1759.0 82 1129.0 83 499.0 84 499.0 85 499.0 86 313.0 87 127.0 88 127.0 89 127.0 90 90.5 91 54.0 92 54.0 93 54.0 94 35.5 95 17.0 96 17.0 97 17.0 98 10.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0014073953936618955 2 0.12485607706629101 3 0.0 4 0.0 5 2.680753130784562E-4 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 5.361506261569124E-4 13 0.0 14 0.0 15 0.0 16 0.0 17 8.712447675049829E-4 18 0.0 19 0.001005282424044211 20 0.0 21 0.0 22 0.0 23 4.6913179788729846E-4 24 6.701882826961406E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1492118.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.50609370775598 #Duplication Level Percentage of deduplicated Percentage of total 1 80.16804314310515 56.52335562215196 2 13.938943941406459 19.65560975439923 3 3.4069420837073734 7.206305334323083 4 1.1449949567856599 3.2291648687215098 5 0.4979122573355 1.7552924136968535 6 0.2408530744457685 1.0188965662004688 7 0.14854344537998304 0.7331252655743813 8 0.08803167957822557 0.49654158796748166 9 0.06052811675587261 0.3840840964749194 >10 0.26796937752468325 3.2529208052133383 >50 0.01941869794850258 0.931981840572317 >100 0.014623488465477725 2.1912380235457727 >500 0.002033651175442899 1.0310732714308517 >1k 0.001162086385967371 1.590410549727755 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3293 0.22069300149183912 No Hit CTGTAGGACGTGGAATATGGCAAGA 3124 0.20936681951427435 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2745 0.1839666836000906 No Hit GTATCAACGCAGAGTACTTTTTTTT 2651 0.1776669137427469 No Hit CTTTAGGACGTGAAATATGGCGAGG 2371 0.15890164182725497 No Hit GGTATCAACGCAGAGTACTTTTTTT 1529 0.1024717884242399 No Hit TATCAACGCAGAGTACTTTTTTTTT 1514 0.10146650600019569 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.701882826961407E-5 0.0 2 0.0 0.0 0.0 6.701882826961407E-5 0.0 3 0.0 0.0 0.0 6.701882826961407E-5 0.0 4 0.0 0.0 0.0 6.701882826961407E-5 0.0 5 0.0 0.0 0.0 6.701882826961407E-5 0.0 6 0.0 0.0 0.0 6.701882826961407E-5 0.0 7 0.0 0.0 0.0 6.701882826961407E-5 0.0 8 0.0 0.0 0.0 6.701882826961407E-5 0.0 9 0.0 0.0 0.0 6.701882826961407E-5 0.0 10 0.0 0.0 0.0 6.701882826961407E-5 0.0 11 0.0 0.0 0.0 6.701882826961407E-5 0.0 12 0.0 0.0 0.0 6.701882826961407E-5 0.0 13 0.0 0.0 0.0 6.701882826961407E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAGG 50 4.514073E-6 17.118994 1 AGACCGG 40 0.005283545 14.24814 6 TAGGACC 510 0.0 13.410015 4 GTCCTAT 165 0.0 12.680736 1 AAGACCG 55 0.0030713468 12.089333 5 GGTATCA 625 0.0 11.869169 1 TCGAACT 75 2.0762242E-4 11.398513 19 AGGACCT 1080 0.0 11.34574 5 ACCGTGG 85 5.329377E-5 11.175013 8 TGGACCG 60 0.0058811815 11.081887 5 GGACCTG 990 0.0 11.033914 6 TGTAGGA 1600 0.0 10.63993 2 TCCAACG 90 9.5261734E-5 10.554177 18 GTCTTAG 190 9.094947E-12 10.5116625 1 ATAAGAC 145 1.445369E-8 10.481391 3 GTAGGAC 1640 0.0 10.425468 3 TAGTACC 75 0.002650333 10.132011 4 TAAGACG 85 6.6005293E-4 10.057511 4 GTATCAA 1270 0.0 10.034756 1 CTAGGGC 95 1.645889E-4 9.998694 3 >>END_MODULE