FastQCFastQC Report
Thu 26 May 2016
SRR1511926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492118
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT38610.2587596959489799No Hit
CTGTAGGACGTGGAATATGGCAAGA32840.22008983203741259No Hit
GTATCAACGCAGAGTACTTTTTTTT32070.21492938226065228No Hit
GTCCTAAAGTGTGTATTTCTCATTT29880.20025225886960682No Hit
CTTTAGGACGTGAAATATGGCGAGG27640.18524004133721328No Hit
GGTATCAACGCAGAGTACTTTTTTT19360.12974845152997283No Hit
TATCAACGCAGAGTACTTTTTTTTT18340.1229125310464722No Hit
GTCCTACAGTGTGCATTTCTCATTT16760.11232355617987316No Hit
CTGAAGGACCTGGAATATGGCGAGA15490.10381216498963218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG400.00526495614.2557115
GGACCGC400.005281381314.249026
TAGGACC5250.013.3958424
GGCGAGG6950.012.70922519
GTCTTAT1154.954927E-912.450981
GTACTAA1302.4247129E-911.7486171
GGTATCA6950.011.5373111
GTTATAA2100.011.363991
TACATCG600.005857972411.0877745
TAGGACA4900.011.05544954
GTATCAA15500.011.0238041
TGTAGGA18750.010.8976992
TGGCGAG18400.010.68497418
CTGTAGG18650.010.64621
AGGACCT11750.010.5957345
GGACCTG11250.010.5548296
GTAGGAC17550.010.5056333
GTGGTAT3000.010.5003271
TGGTATC2650.010.4003932
GGACGTG17850.010.2710026
Produced by FastQC (version 0.11.5)