FastQCFastQC Report
Thu 26 May 2016
SRR1511925_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511925_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583828
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20420.1289281411870481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6150.012.2167321
TCGAACT951.36206E-510.99859119
TAGGACC4850.010.9676694
GGTCTAT1053.4201985E-610.8691361
TGGCGAG8500.010.7280418
CTCGAAC909.526768E-510.55420518
GTATCAA13100.010.4542841
GTCCTAA4650.010.4308651
TGTAGGA11600.010.4120232
GTAGAAC2650.010.39489653
GGCGAGG3700.010.26895619
GTAGGAC11900.010.217183
AGGACGT9550.010.1452995
AGGACCT7250.010.0883655
GGACGTG9250.010.0635786
CTGTAGG11550.010.0457161
GGCGAGA5700.09.9987219
TAGGGTC1054.1101175E-59.9511075
GTCCTAC10550.09.9161551
CGTGGAA5250.09.7701799
Produced by FastQC (version 0.11.5)