FastQCFastQC Report
Thu 26 May 2016
SRR1511925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583828
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25750.16258078528729128No Hit
GTCCTACAGTGGACATTTCTAAATT17980.11352242794040766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCC604.1121742E-412.65932114
TAGGACC4700.012.5369184
CCAACGA701.0907216E-412.2145319
GGCGTGT550.00308257812.0835168
GTCTTAG2000.011.9336711
GGTATCA6850.011.7071921
TGTAGGA12000.011.2461552
GTAGGAC10650.011.1546863
ATCCCGT700.001499453110.85016210
GTCTTAC1501.9645086E-910.8198611
CTCGAAC1252.2388667E-710.64021218
TAGGACA4050.010.5597694
TGGCGAG9800.010.46959718
TCCAACG1106.042843E-610.36384318
GCCTCGA1106.083974E-610.35795416
TCGAACT1201.5229016E-610.29187219
GACGTGG4250.010.2760667
CTGTAGG11350.010.2619051
GGCGAGG3800.010.25020519
TAGACCC750.00263886510.1373795
Produced by FastQC (version 0.11.5)