FastQCFastQC Report
Thu 26 May 2016
SRR1511924_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511924_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463280
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24380.16661199497020393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG802.8702621E-511.8733135
TGACCCG658.032498E-411.6906465
GGTATCA5750.011.5778161
GTATCAA13250.011.1253141
GTACCGT803.7753434E-410.6859826
TTAATAC1701.382432E-1010.6161393
GTCCTAC7000.010.5972471
GTAGGAC7400.010.5255323
GTATAAC1303.782261E-710.2419151
TGTAGGA7300.010.1617442
GGACGTG7950.010.155796
GCGTAGA750.002623606510.1443731
TAGGACG8100.010.0849864
TAGGACC1901.0004442E-109.9985794
TGCCGGT951.6460315E-49.99857910
AGAGCGC1054.1101746E-59.9509678
AGGACGT7950.09.916835
GTCTTAG2400.09.9066141
TAAGGTG1356.4512096E-79.8504525
TCCTACA8600.09.8421052
Produced by FastQC (version 0.11.5)