FastQCFastQC Report
Thu 26 May 2016
SRR1511924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463280
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29160.19927833360669184No Hit
TATCAACGCAGAGTACTTTTTTTTT17480.1194576567710896No Hit
GGTATCAACGCAGAGTACTTTTTTT17070.11665573232737413No Hit
GTCCTACAGTGGACATTTCTAAATT14660.10018588376797333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6400.013.2706141
GTATTAG1458.913048E-1111.8463751
GTCCTAC7550.010.9964691
CTTATAC1502.1154847E-910.7712793
TATACTG2800.010.5223645
TAAACTG1553.6325218E-910.423825
TCCTACA7850.010.4121032
GGACCAT1752.3646862E-1010.3127086
GTATCAA13650.010.2769831
TTAGACA2053.6379788E-1210.1994914
TTAGGAC5150.010.1499793
TAGGACC2250.010.1376744
GGCGTGC951.6524688E-49.9947128
CCTAGAC1356.40106E-79.8560733
CCTATAC1356.40106E-79.8560733
GATATAC3700.09.800831
GGACGTG7750.09.682396
ACAGTGC2850.09.6615558
AGGACGT8100.09.6214055
CCTACAG8250.09.561673
Produced by FastQC (version 0.11.5)