FastQCFastQC Report
Thu 26 May 2016
SRR1511923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026452
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24230.2360558506388998No Hit
GGTATCAACGCAGAGTACTTTTTTT14920.14535506774793172No Hit
TATCAACGCAGAGTACTTTTTTTTT14290.139217420785385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGG350.002171235216.28379210
GTCTGCG400.005281619314.2483199
GGCGTGC905.4066913E-712.6651748
GGTATCA5400.012.1510721
GCGTAGA550.00304426612.1030441
AGACCGG550.003069935412.08948410
TCTTATA1701.0913936E-1111.1876872
TGATACT700.00149212310.8558624
CCTATAC700.00149212310.8558623
GCGTGCG803.7724338E-410.6862399
CTAGACA1002.3993163E-510.4487684
TAGGCTG1659.276846E-1010.3624145
GTCTAAG1502.401066E-810.1435021
ACTAGGG750.002624330610.1435022
CGTGCGC750.002648852810.13213810
TAGAATA750.002648852810.1321384
GTGCGCC750.002648852810.13213811
GTAGAAC951.6445442E-49.9988213
ATAATAC1054.1058356E-59.9512073
AAGGCGT1054.1058356E-59.9512076
Produced by FastQC (version 0.11.5)