FastQCFastQC Report
Thu 26 May 2016
SRR1511923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026452
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29670.2890539450456524No Hit
GGTATCAACGCAGAGTACTTTTTTT17950.17487422694875163No Hit
TATCAACGCAGAGTACTTTTTTTTT17220.1677623503096102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.013.7970011
CGAGTGG550.003079858612.08427414
GTGCGAT550.003082106812.083096511
GCGCCAC752.0830985E-411.39374413
ACTATAC855.2981406E-511.1806873
GTACTAG600.00566406211.1374781
ACTGGCG600.005899535511.0767138
CTAGGAT700.001485969810.8612394
GTATCAA14800.010.7719621
CTTAAAC803.7552055E-410.6915333
GCGTGCG803.7891866E-410.6811169
CCCTTAG1002.270682E-510.5010511
TGCTAGA1002.3862374E-510.4539422
AAGGCGT1002.3991119E-510.4488476
CTTATAC1106.0104594E-610.3675463
GTATAGT856.300094E-410.1079631
GTCTTAT1053.886636E-510.0010011
GTCATAG1252.4108176E-69.9282671
GTATATA1951.4915713E-109.791191
GTCCTAG900.00106430439.546411
Produced by FastQC (version 0.11.5)