FastQCFastQC Report
Thu 26 May 2016
SRR1511922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821212
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19920.24256830148609615No Hit
GGTATCAACGCAGAGTACTTTTTTT12790.155745410442127No Hit
TATCAACGCAGAGTACTTTTTTTTT12240.1490479924794085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGC601.4671059E-615.8308988
GTACCGT456.765411E-414.7755046
GTGCGCC602.5690755E-514.24780811
CGTGCGC500.001500234813.29795510
ATAGATC500.001500234813.2979553
CTAGTAG500.001500234813.2979553
GGTATCA4650.013.2969581
GCGCCAC701.0912588E-412.21240713
CTATCAT550.003036418512.106741
TGAGTAC550.003069862712.0890483
GTGGTAT1052.667948E-711.7773051
ATTACAC600.00587838711.0816293
CTAATAC600.00587838711.0816293
ATAAGCC1303.2629032E-810.9598533
TATACTG1053.4655004E-610.8554735
ACTGGTA700.001492018810.8554738
TTAAGCT803.7719513E-410.6858574
AAAGGCG803.7719513E-410.6858575
CTACACC1201.5229361E-610.2900844
CTAGGAC750.002648675810.1317753
Produced by FastQC (version 0.11.5)