FastQCFastQC Report
Thu 26 May 2016
SRR1511922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821212
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24590.29943546855136066No Hit
TATCAACGCAGAGTACTTTTTTTTT15780.19215500991217857No Hit
GGTATCAACGCAGAGTACTTTTTTT15250.18570113442083166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4700.014.4093681
GGCGTGC400.0052924814.2434238
TAAGGTA551.9518894E-413.8235884
GTAATAC600.00585457911.0876713
CTAATGC600.00585457911.0876713
CAGCGTC700.001495854210.8521329
ATTGCCT700.001496614310.85147210
GTGGTAT1855.456968E-1210.8275951
CCTAGAC803.7529954E-410.6916823
TACAACT803.7529954E-410.6916824
ACTGGTC909.546187E-510.5506848
CTAGAGC1002.3842722E-510.4540893
TATGGGC750.002636723210.1372993
TAGGACT750.002636723210.1372994
TGGTATC1502.4207111E-810.1372992
ACAGCGC856.6138967E-410.0541818
GTATTGT951.575842E-410.0406271
GTCTTAC951.575842E-410.0406271
TTATGGT951.6352077E-410.0039134
GTTGTAG1053.919175E-59.9928141
Produced by FastQC (version 0.11.5)