##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511922_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 821212 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.345765770592735 31.0 31.0 34.0 28.0 34.0 2 31.540751961734607 33.0 31.0 34.0 28.0 34.0 3 31.664047773291184 34.0 31.0 34.0 28.0 34.0 4 35.24722118040165 37.0 35.0 37.0 32.0 37.0 5 34.94233523134099 37.0 35.0 37.0 32.0 37.0 6 34.918124674261946 37.0 35.0 37.0 32.0 37.0 7 34.807422443899995 37.0 35.0 37.0 32.0 37.0 8 34.70773929265525 37.0 35.0 37.0 32.0 37.0 9 36.246935017997785 39.0 37.0 39.0 32.0 39.0 10 36.06301904989211 39.0 37.0 39.0 32.0 39.0 11 36.10234019960741 39.0 37.0 39.0 32.0 39.0 12 35.882821975324276 39.0 37.0 39.0 31.0 39.0 13 35.869133670720835 39.0 37.0 39.0 31.0 39.0 14 36.92882714816637 40.0 37.0 41.0 31.0 41.0 15 36.8145972051066 40.0 37.0 41.0 31.0 41.0 16 36.715871175774346 40.0 37.0 41.0 31.0 41.0 17 36.62779038786574 40.0 36.0 41.0 31.0 41.0 18 36.773723472136304 40.0 37.0 41.0 31.0 41.0 19 36.83201658037145 40.0 37.0 41.0 31.0 41.0 20 36.752629040978455 40.0 37.0 41.0 31.0 41.0 21 36.66313083588647 40.0 37.0 41.0 31.0 41.0 22 36.54371952674827 40.0 37.0 41.0 30.0 41.0 23 36.41597297652738 40.0 37.0 41.0 30.0 41.0 24 36.260079005177715 40.0 36.0 41.0 30.0 41.0 25 35.454485565237725 39.0 35.0 40.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09763337925033255 1101 2 0.07711450863982705 1101 3 0.14214312967609288 1101 4 0.06074186452651276 1101 5 -0.17393490787338095 1101 6 -0.06224882973101842 1101 7 -0.17659213743539937 1101 8 -0.3361747045633976 1101 9 -0.20188911890254246 1101 10 -0.21948506534447887 1101 11 -0.47988588748079053 1101 12 -0.16982581521176598 1101 13 -0.2169332216788291 1101 14 -0.41903970387730993 1101 15 -0.2804574489539178 1101 16 -0.4188248600524034 1101 17 -0.3219115761804261 1101 18 -0.6085912599230525 1101 19 -0.5691550571375501 1101 20 -0.6207289239379321 1101 21 -0.6789196280859358 1101 22 -0.556814560541504 1101 23 -0.6391247847816643 1101 24 -0.7236118915110268 1101 25 -0.8399202417274978 1102 1 -0.032097265923372476 1102 2 -0.15408723419906067 1102 3 -0.17750614709195744 1102 4 0.016710548852515217 1102 5 -0.09558875728409078 1102 6 -0.08796648059358603 1102 7 -0.10709874621272064 1102 8 0.07747394096441695 1102 9 0.03404076724327609 1102 10 0.21035874118808806 1102 11 0.2731698541110106 1102 12 0.05780865050495976 1102 13 0.1114025365407656 1102 14 -0.016393113729279207 1102 15 0.14830401152043748 1102 16 0.3002362855716356 1102 17 0.1120953366699311 1102 18 0.18569524616225408 1102 19 0.2542116087593058 1102 20 0.29544391271711135 1102 21 0.25029216840837165 1102 22 0.25203973843542826 1102 23 0.2516460211273639 1102 24 0.15734389857850317 1102 25 0.740107933141438 1103 1 0.40487463407182034 1103 2 0.2508039194209566 1103 3 0.4604904286937419 1103 4 0.3117365632667557 1103 5 0.21234832000478576 1103 6 0.6279808915253255 1103 7 0.6481660757170431 1103 8 0.6256163231969651 1103 9 0.5798756875729794 1103 10 0.6925406239142973 1103 11 0.9312106562498528 1103 12 1.0897877058507675 1103 13 0.9056778862654582 1103 14 1.0579596690919644 1103 15 1.4563834562504425 1103 16 1.4082210651979281 1103 17 1.2790479708373752 1103 18 1.1506118410288906 1103 19 0.9476778676757647 1103 20 1.297819784948807 1103 21 1.0325550341725105 1103 22 1.3857893270489896 1103 23 1.0878014346030866 1103 24 1.3883396941166666 1103 25 1.3886267570754427 1104 1 0.014005377362018123 1104 2 0.10789557066489408 1104 3 0.04458880254069797 1104 4 0.045213516954675015 1104 5 -0.049167520905797346 1104 6 0.004592962950241031 1104 7 -0.06889572980295355 1104 8 0.020059020138504025 1104 9 0.14828676852950196 1104 10 0.12734819946128795 1104 11 0.24483581443950442 1104 12 0.19509263697883483 1104 13 0.1416822168214793 1104 14 0.24807619908764877 1104 15 0.3915787336035379 1104 16 0.2184556613662636 1104 17 0.46083622713021555 1104 18 0.3953650651717453 1104 19 0.18521471763516217 1104 20 0.45806864561755134 1104 21 0.2620415853089142 1104 22 0.4683259209788986 1104 23 0.08570199274738144 1104 24 0.2760561304787075 1104 25 0.37135939644692684 1105 1 0.049497709888250085 1105 2 0.08376320585456298 1105 3 -0.01277600779021526 1105 4 0.047332161022474395 1105 5 0.029928524291939596 1105 6 0.05812403639656338 1105 7 -0.08265682746235825 1105 8 0.21559977881485537 1105 9 -0.15135003534459202 1105 10 -0.13911669157987916 1105 11 -0.15028121542329131 1105 12 -0.16241341628992956 1105 13 -0.23380204467972732 1105 14 -0.2578761818647308 1105 15 0.013612631908621609 1105 16 -0.06215370102275841 1105 17 0.07074341034248732 1105 18 -0.3042112060146991 1105 19 -0.047614176634411365 1105 20 -0.05540996616614535 1105 21 -0.08684864875080933 1105 22 0.17135255536146587 1105 23 -0.06324877399999451 1105 24 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-0.41833717477253174 2314 15 -0.46526274803058953 2314 16 -0.32266535249185324 2314 17 -0.5547631752704874 2314 18 -0.48285862242807553 2314 19 -0.36020282911668033 2314 20 -0.4930492772097068 2314 21 -0.3894575646575973 2314 22 -0.3856383260974994 2314 23 -0.34830449841741995 2314 24 -0.45212119165822173 2314 25 -0.8021041036303487 2315 1 -0.4854208941961673 2315 2 -0.6310976412478411 2315 3 -0.5837305820457104 2315 4 -0.5929844057270515 2315 5 -0.6248699355218719 2315 6 -0.4770650750719696 2315 7 -0.8794277978771348 2315 8 -0.9327564623510014 2315 9 -0.6187794840944036 2315 10 -0.6879323078980519 2315 11 -1.0733133351289013 2315 12 -1.0342587153102585 2315 13 -1.0480694814394909 2315 14 -1.093128655423243 2315 15 -0.9130871780051137 2315 16 -1.4334299597350437 2315 17 -1.2250369181655856 2315 18 -1.3984099192768724 2315 19 -0.9705841527517762 2315 20 -1.279731260702384 2315 21 -1.297779827773084 2315 22 -1.4589369606496518 2315 23 -1.1535811812720098 2315 24 -1.4722468477047386 2315 25 -1.2856860631677947 2316 1 -0.7985278417958384 2316 2 -0.8703889428097824 2316 3 -0.8456157290445709 2316 4 -1.0255482106383838 2316 5 -0.9035790250692699 2316 6 -0.9579038381678942 2316 7 -1.3566696033043968 2316 8 -1.2149751340771289 2316 9 -1.3133709412679337 2316 10 -1.476253741521802 2316 11 -1.5339049088030805 2316 12 -1.3828837995651924 2316 13 -1.5194872943893714 2316 14 -1.5312501656045683 2316 15 -1.2714338256988142 2316 16 -1.364621761417645 2316 17 -1.56450629929747 2316 18 -1.2853668389996002 2316 19 -1.3884678351709923 2316 20 -1.3747479452602178 2316 21 -1.5473887184372188 2316 22 -1.4708657953934718 2316 23 -1.5741953477514414 2316 24 -1.3458852463200444 2316 25 -1.3812686080413528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9162.0 3 0.0 4 177.0 5 1310.0 6 1702.0 7 1499.0 8 1246.0 9 1150.0 10 1115.0 11 1101.0 12 999.0 13 1027.0 14 970.0 15 1021.0 16 965.0 17 1037.0 18 1103.0 19 1271.0 20 1570.0 21 1894.0 22 2549.0 23 3095.0 24 3754.0 25 4548.0 26 5181.0 27 5704.0 28 6904.0 29 8282.0 30 10501.0 31 14026.0 32 18756.0 33 25918.0 34 36378.0 35 54083.0 36 93233.0 37 190871.0 38 307110.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.00656920290165 18.867347600241192 13.263038418401107 24.863044778456047 2 16.13614998319557 20.88693297224103 38.45681748440111 24.520099560162297 3 18.622718615899426 24.735878189797518 30.3631705333093 26.27823266099375 4 13.718625641125556 17.37797304471927 36.878053413734825 32.02534790042036 5 14.047315349163595 37.37459385770309 34.9979837555264 13.58010703760692 6 32.2502400015594 36.96218939899711 17.506956390376548 13.280614209066943 7 28.35528462808629 31.840986249592067 22.0655567624438 17.738172359877836 8 26.368221604165548 35.09288709858112 20.277711480105015 18.261179817148314 9 26.63077561925452 15.499842301833075 19.84719717385401 38.022184905058396 10 15.595120383043598 28.466096452560365 33.65318577906801 22.285597385328025 11 34.64001987306566 22.738708153314857 23.69193338626323 18.92933858735625 12 23.01293697607926 24.965295197829548 30.785351407431943 21.23641641865925 13 28.66896733121289 21.431006853285144 25.83669016039707 24.063335655104893 14 22.142759725868594 20.983010477197116 27.252280775244397 29.621949021689893 15 23.877878063155432 28.125380535111518 23.9844278943805 24.012313507352548 16 23.629586513592105 27.84007052989971 25.370793412663232 23.15954954384495 17 22.83941296522701 27.38513319337759 26.720627560240228 23.054826281155172 18 22.932567960526637 27.18092283113252 27.444435785156575 22.44207342318427 19 23.889804934958022 26.816145900565296 27.404093223992344 21.889955940484338 20 23.94217327559753 26.37455370842121 27.31377524926572 22.369497766715536 21 24.1686556259133 26.293229420360447 27.023258645884074 22.514856307842184 22 24.305525112153493 25.99379441692361 26.990998494878205 22.709681976044696 23 23.947142164730753 26.038000550450224 27.015658603951064 22.99919868086796 24 23.732848299966985 26.35919189802604 26.928854327960362 22.979105474046612 25 23.70080399304773 26.21521582116057 27.08412756218736 22.99985262360434 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 98.0 1 98.0 2 166.5 3 235.0 4 235.0 5 235.0 6 613.0 7 991.0 8 991.0 9 991.0 10 1380.0 11 1769.0 12 1769.0 13 1769.0 14 2713.5 15 3658.0 16 3658.0 17 3658.0 18 6279.5 19 8901.0 20 8901.0 21 8901.0 22 13289.0 23 17677.0 24 17677.0 25 17677.0 26 25074.5 27 32472.0 28 32472.0 29 32472.0 30 41006.5 31 49541.0 32 49541.0 33 49541.0 34 58082.5 35 66624.0 36 66624.0 37 66624.0 38 75101.0 39 83578.0 40 83578.0 41 83578.0 42 91285.0 43 98992.0 44 98992.0 45 98992.0 46 103041.0 47 107090.0 48 107090.0 49 107090.0 50 107205.5 51 107321.0 52 107321.0 53 107321.0 54 99655.0 55 91989.0 56 91989.0 57 91989.0 58 80058.5 59 68128.0 60 68128.0 61 68128.0 62 55768.0 63 43408.0 64 43408.0 65 43408.0 66 33192.0 67 22976.0 68 22976.0 69 22976.0 70 16639.0 71 10302.0 72 10302.0 73 10302.0 74 7048.5 75 3795.0 76 3795.0 77 3795.0 78 2513.0 79 1231.0 80 1231.0 81 1231.0 82 789.5 83 348.0 84 348.0 85 348.0 86 213.0 87 78.0 88 78.0 89 78.0 90 43.5 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4394723895900206 2 0.0 3 0.0 4 0.0 5 0.04615129832491489 6 0.04676015450334384 7 0.0 8 0.0 9 0.0028007384207731985 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.006088561784289562 20 0.0 21 0.001461254828229495 22 0.0019483397709726597 23 0.008036901555262222 24 0.04566421338217171 25 0.022527678601871378 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 821212.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.07913338206502 #Duplication Level Percentage of deduplicated Percentage of total 1 69.36440900627397 39.59260353637223 2 16.631745311599435 18.986512180346374 3 6.413653730011351 10.9825739016509 4 3.0367091219818203 6.933309000645354 5 1.596220582132082 4.555544375735729 6 0.9303490089850763 3.18621091054387 7 0.5462760522562883 2.1826674555115213 8 0.37038302973948845 1.6912913885562892 9 0.25585595653438153 1.3143632642659613 >10 0.8069084325992312 7.070799445112241 >50 0.03481231228983577 1.3367041478618997 >100 0.011173350720612592 1.1510434866665682 >500 8.594885008066515E-4 0.3342134754244026 >1k 6.446163756049886E-4 0.6821634313066155 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2459 0.29943546855136066 No Hit TATCAACGCAGAGTACTTTTTTTTT 1578 0.19215500991217857 No Hit GGTATCAACGCAGAGTACTTTTTTT 1525 0.18570113442083166 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 470 0.0 14.409368 1 GGCGTGC 40 0.00529248 14.243423 8 TAAGGTA 55 1.9518894E-4 13.823588 4 GTAATAC 60 0.005854579 11.087671 3 CTAATGC 60 0.005854579 11.087671 3 CAGCGTC 70 0.0014958542 10.852132 9 ATTGCCT 70 0.0014966143 10.851472 10 GTGGTAT 185 5.456968E-12 10.827595 1 CCTAGAC 80 3.7529954E-4 10.691682 3 TACAACT 80 3.7529954E-4 10.691682 4 ACTGGTC 90 9.546187E-5 10.550684 8 CTAGAGC 100 2.3842722E-5 10.454089 3 TATGGGC 75 0.0026367232 10.137299 3 TAGGACT 75 0.0026367232 10.137299 4 TGGTATC 150 2.4207111E-8 10.137299 2 ACAGCGC 85 6.6138967E-4 10.054181 8 GTATTGT 95 1.575842E-4 10.040627 1 GTCTTAC 95 1.575842E-4 10.040627 1 TTATGGT 95 1.6352077E-4 10.003913 4 GTTGTAG 105 3.919175E-5 9.992814 1 >>END_MODULE