FastQCFastQC Report
Thu 26 May 2016
SRR1511921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424277
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25730.18065306116717464No Hit
TATCAACGCAGAGTACTTTTTTTTT14860.10433363734722952No Hit
GGTATCAACGCAGAGTACTTTTTTT14610.10257836081043224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG456.768849E-414.7757594
GGTATCA6550.013.2138331
GTATAGG1302.566594E-911.70591451
GTCCTAT1104.8923357E-711.2403381
CGTGCGC700.001492971710.85565910
TCGAACT1053.4706245E-610.85565919
CTAAGGT803.775025E-410.6860394
TCCAACG909.5260664E-510.55411318
GTGCTAC1002.3674003E-510.4621611
GTGCGCC1002.4016072E-510.44857111
GTATCAA13100.010.1644871
CCAACGA856.6005177E-410.05744719
CCGTTTC856.6005177E-410.05744713
GTAGGAC7750.010.0502393
TAGGACG7950.010.0363644
AGGACGT7500.010.0052995
GTCCTAC7350.09.96396351
CTAGAAC1252.5729314E-69.8786493
TTTAGAC1451.6189551E-79.8262423
TAGACTG1451.6189551E-79.8262425
Produced by FastQC (version 0.11.5)