FastQCFastQC Report
Thu 26 May 2016
SRR1511921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424277
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30010.21070339547714384No Hit
TATCAACGCAGAGTACTTTTTTTTT17950.12602885534204372No Hit
GGTATCAACGCAGAGTACTTTTTTT16910.1187269049489671No Hit
GTCCTACAGTGGACATTTCTAAATT15190.1066506023758019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3300.013.2471284
GGTATCA5700.012.7261731
GGCGAGG3800.011.50085119
GTATCAA13700.011.356021
GTAGGAC8850.011.1678063
GTCCTAC6950.010.8492921
GTCTAAT803.610412E-410.7377081
GTCCTAA4900.010.7133611
TATGTCG803.787295E-410.68228616
TGTAGGA9900.010.5593042
TCTATAC2100.010.4084573
ACCGTGC1106.08329E-610.3578528
CTGTAGG9800.010.3237841
AGGACGT8500.010.2860055
TAGGACG8600.010.2769034
GGACGTG8050.010.2667586
TTAGGAC5100.010.2487363
GTAGAAC3250.010.2344033
AGAACCG750.00263955110.1369315
TAGAACA3700.010.017074
Produced by FastQC (version 0.11.5)