FastQCFastQC Report
Thu 26 May 2016
SRR1511920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355128
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16750.12360455986445561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG400.005282654414.2483689
ATGACCG400.005282654414.2483685
GGTATCA5450.012.7374121
CGTCTCG658.0299744E-411.690968517
ACTAGGC658.0299744E-411.69096858
CTAGAAC1406.8030204E-1011.5343923
ATAAGAC1104.973135E-711.22598653
CTAGATA855.327676E-511.175194
GCACCGT855.327676E-511.175196
AACCGTC700.001492614910.8558997
GGCGTGC700.001492614910.8558998
CCTACAC1408.358256E-910.8558993
GTGTAGC2300.010.3363461
CTAAGAC1409.6953954E-810.1774053
ATTATAC1502.4463588E-810.1321733
CCCTTAT856.531873E-410.0688161
TACACGG856.5987307E-410.0576715
TTTACCC951.6453708E-49.9988553
GACGTGA951.6453708E-49.9988557
GTCCTAC2201.8189894E-129.9416851
Produced by FastQC (version 0.11.5)