FastQCFastQC Report
Thu 26 May 2016
SRR1511920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355128
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20630.15223654149275934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCG307.757318E-418.9871229
TAAACCG400.0052571714.2587565
GGTATCA5200.013.5847531
TAAGCTG801.9851195E-613.0705265
TAGTACT751.4689429E-512.674454
ACCGTTG550.00308371512.08271412
ACCGTGT1052.7426904E-711.7539328
GTATTAC1104.6648165E-711.2816881
ACCGTCT1105.009497E-711.2196638
CGTTGCT600.005901477311.07663914
ATCCACG600.005904719711.07582211
GACCGTC700.001499646310.8497847
GGACCGT803.7704737E-410.6873556
GATCGTG803.793694E-410.6802577
AACAGCG750.002662126210.1264667
GTATCAA11150.010.1025391
CGGAGAC951.633311E-410.0061453
AGGACGT2002.5465852E-119.981135
GTATTAT1659.429641E-99.8353181
TATACAG3000.09.8226995
Produced by FastQC (version 0.11.5)