FastQCFastQC Report
Thu 26 May 2016
SRR1511919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913419
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28900.31639368132259127No Hit
GGTATCAACGCAGAGTACTTTTTTT18240.19968929921536557No Hit
TATCAACGCAGAGTACTTTTTTTTT15750.17242908238168903No Hit
GTCCTACAGTGGACATTTCTAAATT9680.1059754614256984No Hit
CTGTAGGACGTGGAATATGGCAAGA9500.10400484334133622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG602.5687646E-514.24835818
GGTATCA5500.013.4859391
GTCTAAG1102.6993803E-912.967251
GTAGGAC5750.012.059483
CTACACT1603.6379788E-1211.8736314
GACGTGG2600.011.690967
CCTATAC907.4605978E-611.6097733
GCTTAGA1001.9039344E-611.411181
GAACAGT2000.011.3986866
TAGGACG4550.011.2734264
ACGTGGA2700.011.2579618
TAAGACC855.323303E-511.1751834
GTACTAG600.005829821311.0942021
GTTATTC600.005877556311.0820563
CTAATTG600.005877556311.0820563
ATTAGAC700.001491816110.8558913
GGACGTG4650.010.826716
AGGACGT4750.010.7987565
TAGGACC2600.010.5949334
TGTAGGA6100.010.4446582
Produced by FastQC (version 0.11.5)