FastQCFastQC Report
Thu 26 May 2016
SRR1511919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913419
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36210.39642267130418785No Hit
GGTATCAACGCAGAGTACTTTTTTT22350.24468507880830154No Hit
TATCAACGCAGAGTACTTTTTTTTT19820.21698694684476674No Hit
GTCCTACAGTGGACATTTCTAAATT11620.12721434522382388No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11260.12327310905509957No Hit
CTGTAGGACGTGGAATATGGCAAGA9700.10619441899062751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG453.5404173E-516.886618
ACCGTGC508.75037E-515.1912868
GGTATCA6200.012.4724491
AGCACCG550.003057322712.0958965
CCAACGA550.003069893212.08927219
GTGGTAT1900.012.0591331
ACTATCC658.056531E-411.6856048
GTCCTAC7450.011.4049181
CTAAGCC855.294764E-511.181083
GTAGGAC5250.011.0426473
TATTAGG951.352186E-511.0045372
TCCTACA8550.010.8933792
TAGGAGT1408.272764E-910.8616214
TATAAAG1408.272764E-910.8616212
TGGTATC2100.010.861622
GTCTTAG1158.2595034E-710.7920491
CCTATTG803.7531336E-410.6919073
AATGCCG803.7721833E-410.68605218
GTATCAA13600.010.6699671
GTATAAC909.042068E-510.6075711
Produced by FastQC (version 0.11.5)