FastQCFastQC Report
Thu 26 May 2016
SRR1511918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516294
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31000.2044458396590635No Hit
GTCCTACAGTGGACATTTCTAAATT25340.16711798635356995No Hit
CTGTAGGACGTGGAATATGGCAAGA23630.1558404900368926No Hit
GTCCTAAAGTGTGTATTTCTCATTT20080.1324281438823869No Hit
GGTATCAACGCAGAGTACTTTTTTT18450.12167824973257164No Hit
TATCAACGCAGAGTACTTTTTTTTT18430.12154634919085613No Hit
CTTTAGGACGTGAAATATGGCGAGG17680.11660007887652395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCG250.006036066518.997795
TCCAACG1700.013.41020518
CGTTTAA550.003045364312.1030731
TAGGACC5400.011.9615734
CCAACGA1459.822543E-1111.79173219
GGTATCA7000.011.6831711
ATAGGAC1604.5474735E-1111.2799383
CCGGTTT1451.2187229E-911.13663612
GTCTTAA1553.074092E-1011.0433571
CGGTTTC1553.1286618E-1011.03097513
GCCGGTT1408.361894E-910.8558811
CAGTGCG700.001492813910.855889
GGCGAGG4050.010.78886919
GACGTGG7700.010.7325187
GTAGGAC15600.010.7167023
GCGCCAC909.5250245E-510.55432813
AGGACGT13250.010.5383985
TGCCGGT1451.4451871E-810.4815410
AGGCGTG1002.401348E-510.4487857
GGACGTG13150.010.3295976
Produced by FastQC (version 0.11.5)