FastQCFastQC Report
Thu 26 May 2016
SRR1511918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516294
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36910.24342244973600108No Hit
GTCCTACAGTGGACATTTCTAAATT27660.18241844919257083No Hit
CTGTAGGACGTGGAATATGGCAAGA25580.16870079285415626No Hit
GGTATCAACGCAGAGTACTTTTTTT22190.1463436510333748No Hit
TATCAACGCAGAGTACTTTTTTTTT22050.14542034724136613No Hit
GTCCTAAAGTGTGTATTTCTCATTT21970.14489274507450403No Hit
CTTTAGGACGTGAAATATGGCGAGG20120.132691944965818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1250.015.19978918
CCAACGA1006.6029315E-1014.24980319
TAGGACC4200.013.3498964
ACCGTGC658.05976E-411.6859768
GGTATCA7600.011.4264911
GTAGGAC13850.011.1157933
TATTCCG600.005860296511.0872035
AGGACGT13600.010.9008645
TGTAGGA15100.010.8879062
TGTAGAA5150.010.8872962
TCGAACT700.001491537810.85699219
TTAGGAC7800.010.843533
GGACGTG13800.010.6687346
GTATCAA17400.010.63991
TAGGACG14600.010.5447774
GTGTAGG2550.010.4785931
GACGTGG7900.010.4563617
GTCCTAC14850.010.4105491
GATATAC4400.010.4105491
ACGTGAA7700.010.23471458
Produced by FastQC (version 0.11.5)