FastQCFastQC Report
Thu 26 May 2016
SRR1511917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349277
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT26740.19818021058685503No Hit
CTGTAGGACGTGGAATATGGCAAGA26670.19766141422406222No Hit
GTCCTAAAGTGTGTATTTCTCATTT25540.18928655865326394No Hit
GTATCAACGCAGAGTACTTTTTTTT21890.16223503402192432No Hit
CTTTAGGACGTGAAATATGGCGAGG21740.16112332753022543No Hit
GGTATCAACGCAGAGTACTTTTTTT14000.10375927255856285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG400.00528313614.2481654
TCCAACG903.6434358E-813.72045618
GGTATCA5450.012.9132141
CCGTTTC951.0410422E-611.99845513
TCTATAC951.0410422E-611.9984553
CCCGTTT802.8694822E-511.87347112
TAGGACC3700.011.55256754
AAGACGT855.3284028E-511.1750325
ACCGCCT600.005880610611.0819068
AATGTCC4800.011.0819068
AAATGTC4050.011.02327257
TAGAAAT4750.010.798614
CTATAGT2200.010.7940654
GACGTGG7450.010.7100317
CCAACGA803.7744673E-410.68612419
GTCCTAA7450.010.5954881
TAGTACA909.524441E-510.5541974
AGGACGT14150.010.5392795
TAGAACA3450.010.4624214
GAAATGT4450.010.4593286
Produced by FastQC (version 0.11.5)