FastQCFastQC Report
Thu 26 May 2016
SRR1511917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349277
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT30670.22730692066936592No Hit
CTGTAGGACGTGGAATATGGCAAGA28770.21322530510784662No Hit
GTATCAACGCAGAGTACTTTTTTTT27490.20373874304534947No Hit
GTCCTAAAGTGTGTATTTCTCATTT26610.19721673162738265No Hit
CTTTAGGACGTGAAATATGGCGAGG21630.1603080761029796No Hit
TATCAACGCAGAGTACTTTTTTTTT16370.12132423512740526No Hit
GGTATCAACGCAGAGTACTTTTTTT16360.121250121361292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5350.013.0242551
TCTATAC1207.348717E-1012.6716623
GGCGAGG6400.012.31877319
GATCGTG550.003080348512.0844717
CACCTTT4150.010.98250814
AGGACGT16400.010.8945395
CTTAGAC1053.448169E-610.8614253
TGCACCG700.001486282910.8614255
GGACGTG16200.010.847416
GTCTTAG1803.274181E-1110.6057481
GAATTAC909.065239E-510.6057481
TCCTATA1355.6494173E-810.5597182
TAGGCTC909.474059E-510.5597185
GACGTGG8750.010.525827
TGGCGAG15000.010.51163118
TGTAGGA15250.010.4697022
GACGTGA7900.010.4564547
TCTAGGA1951.4551915E-1110.2348042
TTAGGAC9750.010.2348033
TAGGACG17500.010.2097394
Produced by FastQC (version 0.11.5)