FastQCFastQC Report
Thu 26 May 2016
SRR1511916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385538
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22530.16260831532588785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGT602.5711313E-514.2480776
GGTATCA7000.012.0926531
ACGATAC600.00588101411.0818393
TCGAACT1201.2769124E-711.08183919
ATAATAC1502.1354936E-910.7652153
TACACGG803.774819E-410.6860595
GCCTCGA1252.2427594E-710.63856516
CTCGAAC1252.2427594E-710.63856518
GTGGTAT2600.010.6085081
TTAGGAC2200.010.3622393
CTTATAT1106.052256E-610.3622393
CCCCTAT750.002621794410.1451471
GCCCTAG750.002621794410.1451471
ATTAGCC750.002650224810.1319673
CTAGACA1054.1094194E-59.9510384
TTAGACA1151.0275264E-59.9117074
TATTGGA1356.33896E-79.8629812
GTATCAA15150.09.8563611
TAGAACA3300.09.7865584
TATTATA1856.348273E-109.7677782
Produced by FastQC (version 0.11.5)