FastQCFastQC Report
Thu 26 May 2016
SRR1511916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385538
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28230.20374756953616574No Hit
TATCAACGCAGAGTACTTTTTTTTT17040.12298471784967283No Hit
GGTATCAACGCAGAGTACTTTTTTT16810.12132471285522303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7000.013.6336591
TTAGTAC701.0883766E-412.2171183
CGTGCGC1053.4851273E-610.85182310
GTACTAG1105.721391E-610.4111591
CTCGAAC1106.0414204E-610.36379418
TCGAACT1106.0414204E-610.36379419
GTATCAA14950.010.2776811
GTCCTAA2350.010.1527251
TTAGGAC2201.8189894E-129.9341213
TCTAGAC1451.6101149E-79.8298653
TAGGACG2900.09.8298654
GTGCTAG1502.47539E-79.5435621
GAATAGC800.0043476679.5435611
CTAGACA1304.2230386E-69.5022034
ACAATAC1201.6947537E-59.5022033
TGCTAGA1106.815919E-59.5022032
TAAGGCA1606.603659E-89.5022034
TCCAACG1106.832095E-59.50014518
GGCGTGC1201.7093003E-59.4953458
GCCCCGT800.0045332189.49534414
Produced by FastQC (version 0.11.5)