FastQCFastQC Report
Thu 26 May 2016
SRR1511915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135921
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17780.156524969606161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCT550.003070181712.0895377
CCGTGCA550.003070181712.0895379
CTACACT1406.8030204E-1011.5344054
GGTATCA5150.011.2635451
CCGCTCC600.005878975611.0820749
GGACGGT600.005878975611.0820746
GTCCTAC4600.010.9564671
TTAGGAC2950.010.947913
CTTATAA700.00147863410.867871
CTAAGAC803.7729475E-410.6862873
TCTAGAC1252.2405402E-710.6387923
GTACTAG909.414242E-510.5659841
TAGGACG4300.010.3825424
GGCGAGG2200.010.3624619
CTAGAAC1201.5240857E-610.2904983
CCCTTAT750.002625036510.1433451
GGTCTAG750.002625036510.1433451
TATGACT856.596914E-410.0576824
TCTACAC1803.929017E-1010.0266393
TCAAGAC1901.0004442E-109.9988653
Produced by FastQC (version 0.11.5)