FastQCFastQC Report
Thu 26 May 2016
SRR1511915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135921
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18050.15890189546632205No Hit
GGTATCAACGCAGAGTACTTTTTTT11780.10370439493591543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG250.006018671719.0067863
AGTACCG500.001495084513.304755
TACACTA1001.4344005E-712.3544115
GGTATCA4800.012.3339071
GCGTGCG701.0966472E-412.2068159
TAGGACC1900.011.5041074
GTCCTAC3250.011.1647871
ACAACCG600.005858413411.0872915
TGGACCG600.005858413411.0872915
CGTGCGC600.00590081811.07655410
CAGGGCG600.00590081811.0765549
GTGCTAG1451.33004505E-810.5366411
TGGTATC2001.8189894E-1210.45373252
CTAGACA1106.01373E-610.3673384
CTACACT1502.4265319E-810.1369524
GTGGTAT2550.010.1105271
AGGACCG856.5684307E-410.0624155
TAAACAG1909.822543E-1110.00357155
GGCGTGC1054.129228E-59.9462958
AGGCGTG1054.129228E-59.9462957
Produced by FastQC (version 0.11.5)