FastQCFastQC Report
Thu 26 May 2016
SRR1511914_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511914_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308710
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT30790.2352698458787661No Hit
CTGTAGGACGTGGAATATGGCAAGA29670.2267118001696327No Hit
GTATCAACGCAGAGTACTTTTTTTT25710.19645299569805383No Hit
GTCCTAAAGTGTGTATTTCTCATTT23180.17712098173010063No Hit
CTTTAGGACGTGAAATATGGCGAGG21360.16321415745275883No Hit
GGTATCAACGCAGAGTACTTTTTTT15330.11713825064376372No Hit
TATCAACGCAGAGTACTTTTTTTTT14500.11079612748431662No Hit
GTCCTACAGTGTGCATTTCTCATTT14220.10865661605703326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATC801.2892087E-714.24879613
ACGAAAT801.2892087E-714.24879612
CGGTAGG707.1954E-613.5832571
CCGCTGA500.001500713313.2983689
TAGGACC4950.013.0489044
GGTATCA5800.012.4591251
ACCGTCC550.003070821312.0894258
CTAACAC1406.8030204E-1011.5342993
ATTAGAC752.0757019E-411.3986023
GTAGGAC16000.011.2204983
TCTACAC1451.2187229E-911.1365643
CGTAGGA600.005836842611.0929942
TGTAGGA15350.011.02588752
GTCGAGG803.7727598E-410.68659819
GGCGAGG6950.010.66097119
GGACCTG10100.010.6274096
GTCCTAC15150.010.6065961
AGGACCT10750.010.6033515
CTGTAGG14900.010.5931171
CTAGACA909.523362E-510.554264
Produced by FastQC (version 0.11.5)