FastQCFastQC Report
Thu 26 May 2016
SRR1511914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308710
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT33440.25551879331555505No Hit
CTGTAGGACGTGGAATATGGCAAGA31970.2442863583223174No Hit
GTATCAACGCAGAGTACTTTTTTTT29470.22518357772157316No Hit
GTCCTAAAGTGTGTATTTCTCATTT25680.19622376233084488No Hit
CTTTAGGACGTGAAATATGGCGAGG23600.18033024887102567No Hit
GGTATCAACGCAGAGTACTTTTTTT17060.13035737481947873No Hit
GTCCTACAGTGTGCATTTCTCATTT16890.12905838573862813No Hit
TATCAACGCAGAGTACTTTTTTTTT16590.12676605206653882No Hit
CTGAAGGACCTGGAATATGGCGAGA13380.1022380817751832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC307.747261E-418.9907158
CCGCTTG400.005295657614.2430369
GGTATCA6750.013.7148741
CCGTGCC550.003079251912.0859
TACCGTG550.003079251912.0857
TAGGACC5350.012.0776344
AACCCTT907.4699565E-611.609436
GGCGAGG5800.011.30138319
TGTAGGA17350.011.2822182
AATCCCG1950.011.20479119
GTCCTAG2500.011.0708731
GTAGGAC17800.010.9969933
TCCAACG1303.2663593E-810.96078918
GTCCTAA7650.010.8537961
GACGTGG9250.010.7785157
CTGTAGG18100.010.7038831
GATATAC4050.010.6042841
GAATTAC909.0774614E-510.6042841
AGGACGT16250.010.5840515
GGACGTG16000.010.5672196
Produced by FastQC (version 0.11.5)