FastQCFastQC Report
Thu 26 May 2016
SRR1511913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381266
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21950.15891218635657434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2000.012.3632391
GGTATCA6500.011.9974631
GTCTTAT1603.6379788E-1211.887731
GTCCTAA2650.011.8428711
GTCTAGA1406.675691E-1011.548081
ACTAGAC600.005880514611.0819623
TCTTATA2100.010.8683882
TGGTATC2050.010.6695762
TAGGACC2500.010.6386824
TTAGACT1553.6652636E-910.4180654
CTAAGGC1106.051412E-610.3623533
GTCTTAA1409.529322E-810.1894831
GTCTAGG1502.4019755E-810.14419651
GTCTAAT856.5273343E-410.0696061
CTAGGGG856.599465E-410.0575773
GAATAGG1054.0552797E-59.963051
GAGTATA1151.0128917E-59.923671
TCCAACG1151.027385E-59.91181718
GTATCAA13250.09.9049461
CTAAAGT3100.09.8052384
Produced by FastQC (version 0.11.5)